Changing Pattern of Bacterial Isolates from Blood Samples in Paediatric ICUs

Objective: To observe most frequent bacterial isolate from septicemic children. Setting: Study was conducted in BMSI, JPMC, Karachi with collaboration of tertiary care hospitals of Karachi during July 2010 to December 2010. Method: Blood samples for culture were collected from 105 children admitted in ICU with suspected septicemia. Specimens were inoculated in brain heart infusion broth in ration of 1:5. subcultures were plerformed at 24 hours, 72 hours and on the 7 th day. Isolates were identified by standard methods. Results: A total of 67 organisms isolated out of 105 samples. These include Gram negative (45/67) and Gram positive (22/67). Klebsiella were 18, Staphylococcus aureus were 12, Staphylococcus epidermidis were 10, Proteus mirabilis 7, Enterobacter 5, Pseudomonas 5, Escherichia coli 4, Acinetobacter 3, Citrobacter 2 and Staphylococcus saprophyticus 1. Conclusion: In the view of isolates Klebsiella pneumoniae tends to show a higher frequency in the paediatric sepsis.